Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
LMNB1

Cell type

Cell type Class
Adipocyte
Cell type
Adipose stem cells
NA
NA

Attributes by original data submitter

Sample

source_name
Human primary adipose stem cells
time point
24 Hours (T24)
cell type
ASCs on 24 hours after adipogenic induction
chip antibody
anti-lamin B1 antibody (Abcam ab16048), at 10 microgram per 10e7 cells
replicate
Rep2

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
For ChIP, cells were sonicated to produce chromatin fragments of ~200 base-pairs, lysates were clarified by sedimentation and histone-DNA or Lamin-DNA complexes were isolated from supernatants by ChIP using specific antibodies. RNA was purified from cell lysates using the RNEasy mini kit (Qiagen). ChIP-seq and RNA-seq sequencing libraries were prepared according to Illumina protocols for sequencing on a Nextseq500 or Novaseq (ChIP-seq) or on a Nextseq500 (RNA-seq) at the Norwegian Sequencing Center.

Sequencing Platform

instrument_model
Illumina HiSeq 4000

hg38

Number of total reads
95600563
Reads aligned (%)
98.6
Duplicates removed (%)
11.8
Number of peaks
2319 (qval < 1E-05)

hg19

Number of total reads
95600563
Reads aligned (%)
97.9
Duplicates removed (%)
13.3
Number of peaks
1889 (qval < 1E-05)

Base call quality data from DBCLS SRA